Nanos3 of the frog Rana rugosa: Molecular cloning and characterization

Maho Kodama, Madoka Yoshida, Masami Endo, Tohru Kobayashi, Akira Oike, Shigeki Yasumasu, Masahisa Nakamura

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Nanos is expressed in the primordial germ cells (PGCs) and also the germ cells of a variety of organisms as diverse as Drosophila, medaka fish, Xenopus and mouse. In Nanos3-deficient mice, PGCs fail to incorporate into the gonad and the size of the testis and ovary is thereby dramatically reduced. To elucidate the role of Nanos in an amphibian species, we cloned Nanos3 cDNA from the testis of the R. rugosa frog. RT-PCR analysis showed strong expression of Nanos3 mRNA in the testis of adult R. rugosa frogs, but expression was not sexually dimorphic during gonadal differentiation. In Nanos3-knockdown tadpoles produced by the CRISPR/Cas9 system, the number of germ cells decreased dramatically in the gonads of both male and female tadpoles before sex determination and thereafter. This was confirmed by three dimensional imaging of wild-type and Nanos3 knockdown gonads using serial sections immunostained for Vasa, a marker specific to germ cells. Taken together, these results suggest that Nanos3 protein function is conserved between R. rugosa and mouse.

本文言語English
ページ(範囲)112-120
ページ数9
ジャーナルDevelopment Growth and Differentiation
60
2
DOI
出版ステータスPublished - 2018 2月
外部発表はい

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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