Naive Human Embryonic Stem Cells Can Give Rise to Cells with a Trophoblast-like Transcriptome and Methylome

Jessica K. Cinkornpumin, Sin Young Kwon, Yixin Guo, Ishtiaque Hossain, Jacinthe Sirois, Colleen S. Russett, Hsin Wei Tseng, Hiroaki Okae, Takahiro Arima, Thomas F. Duchaine, Wanlu Liu, William A. Pastor

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Human embryonic stem cells (hESCs) readily differentiate to somatic or germ lineages but have impaired ability to form extra-embryonic lineages such as placenta or yolk sac. Here, we demonstrate that naive hESCs can be converted into cells that exhibit the cellular and molecular phenotypes of human trophoblast stem cells (hTSCs) derived from human placenta or blastocyst. The resulting “transdifferentiated” hTSCs show reactivation of core placental genes, acquisition of a placenta-like methylome, and the ability to differentiate to extravillous trophoblasts and syncytiotrophoblasts. Modest differences are observed between transdifferentiated and placental hTSCs, most notably in the expression of certain imprinted loci. These results suggest that naive hESCs can differentiate to extra-embryonic lineage and demonstrate a new way of modeling human trophoblast specification and placental methylome establishment.

本文言語English
ページ(範囲)198-213
ページ数16
ジャーナルStem Cell Reports
15
1
DOI
出版ステータスPublished - 2020 7 14
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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