NAADP+ initiates the Ca2+ response during fertilization of starfish oocytes

Dmitri Lim, Keiichiro Kyozuka, Giovanni Gragnaniell, Ernesto Carafoli, Luigia Santella

研究成果: Article査読

82 被引用数 (Scopus)

抄録

We have explored the role of the recently discovered second messenger nicotinic acid adenine nucleotide phosphate (NAADP+) in Ca2+ swings that accompany the fertilization process in starfish oocytes. The injection of NAADP+ deep into the cytoplasm of oocytes matured by the hormone 1-methyladenine (1-MA), mobilized Ca2+ exclusively in the cortical layer, showing that the NAADP+-sensitive Ca2+ pool is restricted to the subplasma membrane region of the cell. At variance with this, InsP3 initiated the liberation of Ca2+ next to the point of injection in the center of the cell. The initial cortical Ca2+ liberation induced by NAADP+ was followed by a spreading of the Ca2+ wave to the remainder of the cell and by a massive cortical granule exocytosis similar to that routinely observed on injection of InsP3. A striking difference in the responses to NAADP+ and InsP3 was revealed by the removal of the nucleus from immature oocytes, i.e., from oocytes not treated with 1-MA. Whereas the Ca2+ response and the cortical granule exocytosis induced by NAADP+ were unaffected by the removal of the nucleus, the Ca2+ response promoted by InsP3 was significantly slowed. In addition, the cortical granule exocytosis was completely abolished. When enucleated oocytes were fertilized, the spermatozoon still promoted the Ca2+ wave and normal cortical exocytosis, strongly suggesting that the Ca2+ response was mediated by NAADP+ and not by InsP3. InsP3-sensitive Ca2+ stores may mediate the propagation of the wave initiated by NAADP+ since its spreading was strongly affected by removal of the nucleus.

本文言語English
ページ(範囲)2257-2267
ページ数11
ジャーナルFASEB Journal
15
12
DOI
出版ステータスPublished - 2001 10 16

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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