NAADP mobilizes Ca2+ from a thapsigargin-sensitive store in the nuclear envelope by activating ryanodine receptors

Julia V. Gerasimenko, Yoshio Maruyama, Kojiro Yano, Nick J. Dolman, Alexei V. Tepikin, Ole H. Petersen, Oleg V. Gerasimenko

研究成果: Article査読

181 被引用数 (Scopus)

抄録

Ca2+ release from the envelope of isolated pancreatic acinar nuclei could be activated by nicotinic acid adenine dinucleotide phosphate (NAADP) as well as by inositol 1,4,5-trisphosphate (IP3) and cyclic ADP-ribose (cADPR). Each of these agents reduced the Ca2+ concentration inside the nuclear envelope, and this was associated with a transient rise in the nucleoplasmic Ca2+ concentration. NAADP released Ca2+ from the same thapsigargin-sensitive pool as IP 3. The NAADP action was specific because, for example, nicotineamide adenine dinucleotide phosphate was ineffective. The Ca2+ release was unaffected by procedures interfering with acidic organelles (bafilomycin, brefeldin, and nigericin). Ryanodine blocked the Ca2+-releasing effects of NAADP, cADPR, and caffeine, but not IP3. Ruthenium red also blocked the NAADP-elicited Ca2+ release. IP3 receptor blockade did not inhibit the Ca2+ release elicited by NAADP or cADPR. The nuclear envelope contains ryanodine and IP3 receptors that can be activated separately and independently; the ryanodine receptors by either NAADP or cADPR, and the IP3 receptors by IP3.

本文言語English
ページ(範囲)271-282
ページ数12
ジャーナルJournal of Cell Biology
163
2
DOI
出版ステータスPublished - 2003 10 27

ASJC Scopus subject areas

  • Cell Biology

フィンガープリント 「NAADP mobilizes Ca<sup>2+</sup> from a thapsigargin-sensitive store in the nuclear envelope by activating ryanodine receptors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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