N-acetyl cysteine protects TMJ chondrocytes from oxidative stress

T. Ueno, M. Yamada, Y. Sugita, T. Ogawa

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Temporomandibular joint (TMJ) inflammation is closely associated with oxidative stress. This study tested the potential of N-acetyl cysteine (NAC), an anti-oxidant amino-acid derivative, in alleviating oxidative stress-related damage in TMJ chondrocytes. The inflammatory condition was simulated by the addition of hydrogen peroxide (H2O2) to TMJ-derived chondrocyte cultures. Exposure to H2O2 decreased the cell population by half within 2 days as a result of induced apoptosis and reduced proliferation. Gene expression of aggrecan and collagen II, as well as glycosaminoglycan production, were reduced by more than 70%. These compromised chondrocyte viability and function were fully restored by the addition of NAC to the cultures. NAC reduced the H2O2-elevated intracellular reactive oxygen species to the normal level and increased cellular glutathione reserves. These results indicate that NAC restores oxidative stress-induced cell death and severe functional impairment in TMJ chondrocytes, and warrant in vivo testing to explore its therapeutic potential as an anti-inflammatory agent.

本文言語English
ページ(範囲)353-359
ページ数7
ジャーナルJournal of dental research
90
3
DOI
出版ステータスPublished - 2011 3
外部発表はい

ASJC Scopus subject areas

  • 歯科学(全般)

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