N-acetyl cysteine as an osteogenesis-enhancing molecule for bone regeneration

Masahiro Yamada, Naoki Tsukimura, Takayuki Ikeda, Yoshihiko Sugita, Wael Att, Norinaga Kojima, Katsutoshi Kubo, Takeshi Ueno, Kaoru Sakurai, Takahiro Ogawa

研究成果: Article査読

57 被引用数 (Scopus)

抄録

Bone regeneration often requires cues from osteogenesis-inducing factors for successful outcome. N-acetyl cysteine (NAC), an anti-oxidant small molecule, possibly modulates osteoblastic differentiation. This study investigated the potential of NAC as an osteogenesis-enhancing molecule invitro and invivo. Various concentrations of NAC (0, 2.5, 5.0, and 10m. m) were added to rat bone marrow stromal cell or osteoblastic cell culture in media with or without dexamethasone. The results showed marked enhancement of alkaline phosphatase activity and mineralized matrix formation together with consistent upregulation of bone-related gene markers such as collagen I, osteopontin, and osteocalcin in the osteoblastic culture with addition of 2.5 or 5.0m. m NAC regardless of the presence of dexamethasone. Micro-CT-based analysis and histological observation revealed that addition of NAC to a collagenous sponge implanted in a critical size cortical bone defect (3.0mm×5.0mm) in rat femur yielded acceleration and completion of defect closure, with thick, compact, and contiguous bone after 6 weeks of healing. In contrast, with sponge alone, only sparse and incomplete bone regeneration was observed during the matching healing period. These results indicate that NAC can function as an osteogenesis-enhancing molecule to accelerate bone regeneration by activating differentiation of osteogenic lineages.

本文言語English
ページ(範囲)6147-6156
ページ数10
ジャーナルBiomaterials
34
26
DOI
出版ステータスPublished - 2013 8
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • バイオエンジニアリング
  • セラミックおよび複合材料
  • 生体材料
  • 材料力学

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