Myelin-associated glycoprotein reduces axonal branching and enhances functional recovery after sciatic nerve transection in rats

Koichi Tomita, Tateki Kubo, Ken Matsuda, Kenji Yano, Masaya Tohyama, Ko Hosokawa

    研究成果: Article査読

    27 被引用数 (Scopus)

    抄録

    The mature peripheral nervous system (PNS) generally shows better regeneration of injured axons as opposed to the central nervous system (CNS). However, complete functional recovery is rarely achieved even in the PNS although morphologically good axonal regeneration often occurs. This mainly results from aberrant reinnervation due to extensive branching of cut axons with consequent failure of synchronized movements of the muscles. Myelin-associated glycoprotein (MAG), a well-characterized molecule existing both in the CNS and PNS myelin, is considered to be a potent inhibitor of axonal regeneration especially in the CNS. In the present study, we investigated whether MAG has any effects not only on axonal elongation, but also on axonal branching. We show herein that MAG minimized branching of the peripheral axons both in vitro and in vivo via activation of RhoA. Furthermore, after sciatic nerve transection in rats, focal and temporary application of MAG to the lesion dramatically enhanced the functional recovery. Using double retrograde labeling and preoperative/postoperative labeling of spinal neurons, reduced hyperinnervation and improved accuracy of target reinnervation was confirmed, respectively. In conclusion, as MAG significantly improves the quality of axonal regeneration, it can be used as a new therapeutic approach for peripheral nerve repair with possible focal and temporary application.

    本文言語English
    ページ(範囲)1498-1507
    ページ数10
    ジャーナルGLIA
    55
    14
    DOI
    出版ステータスPublished - 2007 11月 1

    ASJC Scopus subject areas

    • 免疫学

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