Mutant selection in the self-incompatible plant radish (Raphanus sativus L. var. sativus) using two-step TILLING

Kaori Kohzuma, Motoko Chiba, Soichiro Nagano, Toyoaki Anai, Miki U. Ueda, Riichi Oguchi, Kazumasa Shirai, Kousuke Hanada, Kouki Hikosaka, Nobuharu Fujii

    研究成果: Article査読

    2 被引用数 (Scopus)

    抄録

    Radish (Raphanus sativus L. var. sativus), a widely cultivated root vegetable crop, possesses a large sink organ (the root), implying that photosynthetic activity in radish can be enhanced by altering both the source and sink capacity of the plant. However, since radish is a self-incompatible plant, improved mutation-breeding strategies are needed for this crop. TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful method used for reverse genetics. In this study, we developed a new TILLING strategy involving a two-step mutant selection process for mutagenized radish plants: the first selection is performed to identify a BC1M1 line, that is, progenies of M1 plants crossed with wild-type, and the second step is performed to identify BC1M1 individuals with mutations. We focused on Rubisco as a target, since Rubisco is the most abundant plant protein and a key photosynthetic enzyme. We found that the radish genome contains six RBCS genes and one pseudogene encoding small Rubisco subunits. We screened 955 EMS-induced BC1M1 lines using our newly developed TILLING strategy and obtained six mutant lines for the six RsRBCS genes, encoding proteins with four different types of amino acid substitutions. Finally, we selected a homozygous mutant and subjected it to physiological measurements.

    本文言語English
    ページ(範囲)268-276
    ページ数9
    ジャーナルBreeding Science
    67
    3
    DOI
    出版ステータスPublished - 2017

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Genetics
    • Plant Science

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