Mutagenicity of 8-nitroguanosine, a product of nitrative nucleoside modification by reactive nitrogen oxides, in mammalian cells

Kazuyoshi Kaneko, Teruo Akuta, Tomohiro Sawa, Ha Won Kim, Shigemoto Fujii, Tatsuya Okamoto, Hitoshi Nakayama, Hajime Ohigashi, Akira Murakami, Takaaki Akaike

研究成果: Article査読

23 被引用数 (Scopus)

抄録

8-Nitroguanosine is a nitratively modified nucleoside that is formed endogeneously under inflammatory conditions dependent on nitric oxide production, particularly associated with cancer risks. Here, we investigated the mutagenic potential of 8-nitroguanosine in mammalian cells. Treatment with 8-nitroguanosine (10-1000 μM) for 1 h significantly increased (by 6-8 times) the mutation frequency of the xanthine-guanine phosphoribosyltransferase (gpt) gene in AS52 cells without cytotoxic effects. 8-Nitroguanosine treatment induced a G-to-T transversion in gpt gene at position 86. It also significantly increased levels of abasic sites in DNA. These observations suggest that formation of 8-nitroguanosine may contribute to the pathogenesis of inflammation-associated carcinogenesis.

本文言語English
ページ(範囲)239-247
ページ数9
ジャーナルCancer Letters
262
2
DOI
出版ステータスPublished - 2008 4月 18
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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