Multiple promoters in rat acyl-CoA synthetase gene mediate differential expression of multiple transcripts with 5′-end heterogeneity

Hiroyuki Suzuki, Mitsuhiro Watanabe, Takahiro Fujino, Tokuo Yamamoto

研究成果: Article査読

49 被引用数 (Scopus)

抄録

Nucleotide sequence analysis of six independently isolated cDNAs for rat acyl-CoA synthetase (ACS) revealed three forms of ACS mRNA, designated form-A, -B, and -C mRNAs, which differ in their 5′-untranslated regions. Form-A mRNA was preferentially detected in normal and peroxisome-induced livers, whereas form-B mRNA was found in peroxisome-induced livers but not in normal livers and hearts, and form-C mRNA was preferentially found in normal hearts and peroxisome-induced livers. Analysis of two overlapping genomic clones for the rat ACS gene revealed that the three 5′-untranslated regions of the mRNAs are individually encoded by three different exons located within a 20-kilobase genomic fragment. The transcription start sites of the three forms of ACS mRNA were determined and nucleotide sequences of 5′-upstream regions of the three 5′-end exons were determined. The 5′-upstream regions were fused to the chloramphenicol acetyltransferase gene and transcription units of the three forms of ACS mRNAs were determined. These data indicate that the three forms of ACS mRNA with 5′-end heterogeneity are generated by alternative transcription from three promoters in the rat ACS gene.

本文言語English
ページ(範囲)9676-9682
ページ数7
ジャーナルJournal of Biological Chemistry
270
16
DOI
出版ステータスPublished - 1995 4 21

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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