Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus

Masahiko Harata, Yukako Oma, Teruko Tabuchi, Yan Zhang, David J. Stillman, Shigeki Mizuno

    研究成果: Article査読

    52 被引用数 (Scopus)

    抄録

    An increasing number of actin-related proteins (Arps), which share the basal structure with skeletal actin but possess distinct functions, have been found in a wide variety of organisms. Individual Arps of Saccharomyces cerevisiae were classified into Arps 1-10 based on the relatedness of their sequences and functions, where Arp1 is the most similar to actin, and Arp10 is the least similar. While Arps 1-3 and their orthologs in other organisms are localized exclusively in the cytoplasm, Arp4 (also known as Act3) is localized in the nucleus and is involved in transcriptional regulation. Here we examined the more divergent Arps for possible nuclear functions. We show that Arps 5-9 are localized in the nucleus, but Arp10 is not. The nuclear export signals identified in actin are well conserved in the cytoplasmic Arps, Arps 1-3, but less conserved in the nuclear Arps. Gel filtration chromatography experiments show that the nuclear Arps are larger than monomer in size and thus are present in multi-protein complexes. Since nuclear protein complexes containing Arps are found to be responsible for histone acetylation and chromatin remodeling, it is suggested that most of the divergent Arps are involved in the transcriptional regulation through chromatin modulation.

    本文言語English
    ページ(範囲)665-671
    ページ数7
    ジャーナルJournal of biochemistry
    128
    4
    DOI
    出版ステータスPublished - 2000 1月 1

    ASJC Scopus subject areas

    • 生化学
    • 分子生物学

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