Multimodal functional analysis platform: 1. Ultrathin fluorescence endoscope imaging system enables flexible functional brain imaging

Makoto Osanai, Hideki Miwa, Atsushi Tamura, Satomi Kikuta, Yoshio Iguchi, Yuchio Yanagawa, Kazuto Kobayashi, Norihiro Katayama, Tetsu Tanaka, Hajime Mushiake

研究成果: Chapter

抄録

To elucidate the expression mechanisms of brain functions, we have developed an ultrathin fluorescence endoscope imaging system (U-FEIS) that can image cells in the brain at any depth while minimizing the invasion. The endoscope part of U-FEIS consists of a GRIN lens and a 10,000-pixel image fiber with a diameter of 450 μm. The specialized microscope of U-FEIS is within 30 cm square and includes lenses and optical filters optimized for the endoscope. Using U-FEIS, we successfully visualized neurons expressing GFP with single-cell resolution and recorded the multineuronal activities in vitro and in vivo. U-FEIS can also perform imaging and optical stimulation simultaneously. Therefore, U-FEIS should be a powerful optical tool in neuroscience research.

本文言語English
ホスト出版物のタイトルAdvances in Experimental Medicine and Biology
出版社Springer
ページ471-479
ページ数9
DOI
出版ステータスPublished - 2021

出版物シリーズ

名前Advances in Experimental Medicine and Biology
1293
ISSN(印刷版)0065-2598
ISSN(電子版)2214-8019

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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