Multi-modal ¹⁹F NMR probe using perfluorinated cubic silsesquioxane-coated silica nanoparticles for monitoring enzymatic activity

The establishment of ¹⁹F NMR signal regulation and the application of this strategy to develop a multi-modal ¹⁹F NMR probe for monitoring enzymatic activity using nanoparticles as a signal regulator is described; water-soluble perfluorinated cubic silsesquioxane was synthesized and immobilized onto the silica nanoparticles for suppressing the signals; ¹⁹F NMR signals of the probes were recovered by releasing from nanoparticles.