Mouse respiratory cilia with the asymmetric axonemal structure on sparsely distributed ciliary cells can generate overall directional flow

Hironori Ueno, Takuji Ishikawa, Khanh Huy Bui, Kohsuke Gonda, Takashi Ishikawa, Takami Yamaguchi

研究成果: Article査読

21 被引用数 (Scopus)

抄録

Mucociliary clearance on the surface of the tracheal lumen is an important component of lung defense against dust mites and viruses. However, the axonemal structure that achieves effective ciliary motion, and the mechanisms by which discretely distributed ciliary cells generate directional flow are unknown. In this study, we examined individual ciliary motion with 7- to 9-nm spatial precision by labeling the ciliary tip with quantum dots and detected an asymmetric beating pattern. Cryo-electron tomography revealed that the densities of two inner dynein arms were missing from at least 2 doublet microtubules in the axonemal structure. Although the flow directions generated by individual ciliated cells were unsteady and diverse, the time- and space-averaged velocity field was found to be directional. These results indicate that the asymmetric ciliary motion is driven by the asymmetric axonemal structure, and it generates overall directional flow from the lungs to the oropharynx on sparsely distributed ciliated cells. From the Clinical Editor: The authors of this study utilized quantum dots in determining the kinetics of ciliary motion in mouse respiratory cilia with 7- to 9-nm spatial precision.

本文言語English
ページ(範囲)1081-1087
ページ数7
ジャーナルNanomedicine: Nanotechnology, Biology, and Medicine
8
7
DOI
出版ステータスPublished - 2012 10

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 医学(その他)
  • 分子医療
  • 生体医工学
  • 材料科学(全般)
  • 薬科学

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