Mouse myosin X: Molecular architecture and tissue expression as revealed by Northern blot and in situ hybridization analyses

Satoshi Yonezawa, Atsushi Kimura, Seizo Koshiba, Shigeo Masaki, Takao Ono, Atsuko Hanai, Shinichi Sonta, Takashi Kageyama, Takayuki Takahashi, Akihiko Moriyama

研究成果: Article査読

24 被引用数 (Scopus)

抄録

The structure of the coding region of mouse myosin X cDNA was determined. The predicted protein sequence indicated an approximately 240 kDa molecular mass with 2062 amino acids. When aligned with the structure predicted for calf myosin X (GenBank Accession No. U55042), extremely highly conserved pleckstrin homology domains and a myosin tail homology 4 domain were apparent in the tail region, suggesting their importance for myosin X's function. Northern blot analysis revealed the existence of a myosin X mRNA, 8.7 kb in size, in various mouse tissues, while a similar size of human type myosin X mRNA was recognized mainly in the testis. In addition to the adult-type transcripts in mice, a smaller embryo-specific mRNA, 4.8 kb in size, was identified in early to late embryonic stages, suggesting the presence of a shorter myosin X isoform in mouse embryos. In situ hybridization experiments with mouse testis revealed that myosin X mRNA was restricted to Sertoli cells at stages VIII-X of the spermatogenesis cycle, suggesting that myosin X is implicated in the supporting cells during the spermatid morphogenesis. (C) 2000 Academic Press.

本文言語English
ページ(範囲)526-533
ページ数8
ジャーナルBiochemical and biophysical research communications
271
2
DOI
出版ステータスPublished - 2000 5 10
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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