Mouse dexamethasone-induced RAS protein 1 gene is expressed in a circadian rhythmic manner in the suprachiasmatic nucleus

Hirokazu Takahashi, Nanae Umeda, Yoko Tsutsumi, Ryutaro Fukumura, Hajime Ohkaze, Mitsugu Sujino, Gijsbertus Van Der Horst, Akira Yasui, Shin Ichi T. Inouye, Akira Fujimori, Tatsuya Ohhata, Ryoko Araki, Masumi Abe

研究成果: Article査読

48 被引用数 (Scopus)

抄録

We identified the Dexamethasone-induced RAS protein 1 (Dexras1) gene as a cycling gene in the suprachiasmatic nucleus (SCN). Investigation of the whole brain using in situ hybridization demonstrated the localization of the expression of the gene in the SCN, thalamus, piriform cortex and hippocampus. However, rhythmic expression of the gene was observed only in the SCN. The rhythmic change in gene expression during 1 day was approximately five-fold, and the maximum expression was observed during subjective night. Real-time PCR using the SCN, paraventricular nucleus and cortex confirmed these results. Next, we analyzed the expression of the Dexras1 gene in the SCN of cryptochrome (Cry) 1 and 2 double knockout mice. We found that the rhythmic expression disappeared. The results indicate that Dexras1 rhythmicity and levels are dependent upon CRYs. This is the first time that the G protein, which may be involved in the input pathway, has been isolated as a cycling gene in the SCN.

本文言語English
ページ(範囲)1-6
ページ数6
ジャーナルMolecular Brain Research
110
1
DOI
出版ステータスPublished - 2003 1月 31
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞および分子神経科学

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