Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles

Hidetake Miyata, Hirokazu Hotani

研究成果: Article査読

90 被引用数 (Scopus)

抄録

Spherical giant liposomes that had encapsulated skeletal-muscle G-actin were made by swelling a dried lipid mixture of dimyristoyl phosphatidylcholine/cardiolipin, 1:1 (wt/wt), in a solution of G-actin/CaCl2 at 0°C. Polymerization of the encapsulated G-actin into actin filaments was achieved by raising the temperature to 30°C. We observed the subsequent shape changes of the liposomes by dark-field and differential interference-contrast light microscopy. After ≈40 min, which was required for completion of actin polymerization, two shapes of liposome were evident: dumbbell and disk. Elongation of the dumbbell-shaped liposomes was concomitant with actin polymerization. Polarization microscopy showed that actin filaments formed thick bundles in the liposomes and that these filaments lay contiguous to the periphery of the liposome. Localization of actin filaments in the liposomes was confirmed by observation of rhodamine phalloidin-conjugated actin filaments by fluorescence microscopy. Both dumbbell-and disk-shaped liposomes were rigid and kept their shapes as far as actin filaments were stabilized. In contrast, liposomes containing bovine serum albumin were fragile, and their shapes continually fluctuated from Brownian motion, indicating that the actin bundles served as mechanical support for the liposome shapes.

本文言語English
ページ(範囲)11547-11551
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
89
23
DOI
出版ステータスPublished - 1992 12 1
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