Monitoring capillary endothelial cell culture and capillary formation in a microdevice by impedance spectroscopy measurements

A. K. Rissanen, S. Ostrovidov, E. Lennon, V. Senez, J. Kim, B. Kim, K. Sakai Furukawa, T. Ushida, Y. Sakai, T. Fujii

    研究成果: Conference contribution

    2 被引用数 (Scopus)

    抄録

    Capillary endothelial cells (ECs) were cultured on gold electrodes in polydimethlysiloxane (PDMS)/glass microdevices. Our objective was to monitor the culture growth by electrical impedance spectroscopy (EIS) measurements, and to observe whether the formation of capillary-like structures in the cell layer produces a change in the impedance spectra. In our contribution, we present a novel micro-device, which enables long-term cell culture and the monitoring of the cell layer properties. We illustrate the usefulness of EIS measurements in observing several culture conditions (confluence, cell detachment, cell death, capillary formation) by providing impedance measurement curves, as well as cell layer pictures of the culture area.

    本文言語English
    ホスト出版物のタイトル2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
    ページ201-204
    ページ数4
    DOI
    出版ステータスPublished - 2005
    イベント2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
    継続期間: 2005 5 122005 5 15

    出版物シリーズ

    名前2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
    2005

    Other

    Other2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
    国/地域United States
    CityOahu, HI
    Period05/5/1205/5/15

    ASJC Scopus subject areas

    • 工学(全般)

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