Molecular viewing of actin polymerizing actions and beyond: Combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation)

Naoki Watanabe, Sawako Yamashiro, Dimitrios Vavylonis, Tai Kiuchi

    研究成果: Review article査読

    5 被引用数 (Scopus)

    抄録

    Live-cell single-molecule imaging is a powerful tool to elucidate the in vivo biochemistry of cytoskeletal proteins. However, it is often somewhat difficult to interpret how a bulk population of the observed molecule might behave as a whole. We review our recent studies in which the combination of image analysis with modeling and bulk kinetics measurements such as FRAP (fluorescence recovery after photobleaching) clarified basic problems in the regulation of actin remodeling pathways. Development, Growth & Differentiation

    本文言語English
    ページ(範囲)508-514
    ページ数7
    ジャーナルDevelopment Growth and Differentiation
    55
    4
    DOI
    出版ステータスPublished - 2013 5

    ASJC Scopus subject areas

    • 発生生物学
    • 細胞生物学

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