Molecular mechanisms of kinetochore capture by spindle microtubules

Kozo Tanaka, Naomi Mukae, Hilary Dewar, Mark Van Breugel, Euan K. James, Alan R. Prescott, Claude Antony, Tomoyuki U. Tanaka

研究成果: Article査読

212 被引用数 (Scopus)

抄録

For high-fidelity chromosome segregation, kinetochores must be properly captured by spindle microtubules, but the mechanisms underlying initial kinetochore capture have remained elusive. Here we visualised individual kinetochore-microtubule interactions in Saccharomyces cerevisiae by regulating the activity of a centromere. Kinetochores are captured by the side of microtubules extending from spindle poles, and are subsequently transported poleward along them. The microtubule extension from spindle poles requires microtubule plus-end-tracking proteins and the Ran GDP/GTP exchange factor. Distinct kinetochore components are used for kinetochore capture by microtubules and for ensuring subsequent sister kinetochore bi-orientation on the spindle. Kar3, a kinesin-14 family member, is one of the regulators that promote transport of captured kinetochores along microtubules. During such transport, kinetochores ensure that they do not slide off their associated microtubules by facilitating the conversion of microtubule dynamics from shrinkage to growth at the plus ends. This conversion is promoted by the transport of Stu2 from the captured kinetochores to the plus ends of microtubules.

本文言語English
ページ(範囲)987-994
ページ数8
ジャーナルNature
434
7036
DOI
出版ステータスPublished - 2005 4 21
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ASJC Scopus subject areas

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