Optic rectum derives from the midbrain alar plate in early chick development. To know the molecular mechanism defining rectum territory along the anteroposterior (AP) end dorsoventral (DV) axis in neural development, we have carried out the gain-of-function experiments using a newly developed gene transfer system, in ovo electroporation. Misexpression of Otx2/Gbx2 and Shh changed the expression of rectum-related genes, resulting in the fate change of the tectum structure along the AP axis end the DV axis, respectively. We conclude that these genes are involved in the establishment of rectum territory in embryogenesis.
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