Molecular mechanism of nitric oxide reduction catalyzed by fungal nitric oxide reductase

Eiji Obayashi, Hideaki Shimizu, Sam Yong Park, Hiro Nakamura, Satoshi Takahashi, Hirofumi Shoun, Yoshitsugu Shiro

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Nitric oxide reductase (NOR) isolated from Fusarium oxysporum catalyzes the reduction reaction of nitric oxide (NO) to nitrous oxide (N 2 O). In this reaction, the transient reaction intermediate (t=∼20 s) is produced by the reduction of ferric-NO complex with NADH. To elucidate the electronic structure of the intermediate, we measured its resonance Raman spectrum using the continuous-flow method. From these spectral features, we have concluded that the electronic structure of the intermediate in the NO reduction reaction by fungal NOR is Fe 2+ NO H + . Further, we have found that the Ser286/Asp393 hydrogen-bond network is the proton pathway to the active site on the basis of crystallographic and mutagenesis studies. From these results, we discuss the molecular mechanism of NO reduction reaction catalyzed by fungal NOR.

本文言語English
ページ(範囲)59-62
ページ数4
ジャーナルInternational Congress Series
1233
C
DOI
出版ステータスPublished - 2002 11月 1
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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