Molecular mapping of the fertility restorer gene for ms-CW-type cytoplasmic male sterility of rice

Sota Fujii, Kinya Toriyama

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Cytoplasmic male sterility (CMS) of rice (Oryza sativa L.) was first reported using the cytoplasm of a Chinese wild rice, Oryza rufipogon Griff. strain W1. However, it was not possible to characterize this ms-CW-type CMS in more detail until a restorer line had been developed due to the lack of restorer genes among cultivars thus far tested. The breeding of a restorer line (W1-R) was eventually achieved by transferring the restorer gene(s) of W1 to a cultivar. We report here the characterization of the ms-CW pollen grains and mapping of the restorer gene for ms-CW-type CMS. Pollen grains of the male-sterile plants appeared to be normal and viable based on the fluorochromatic reaction test, but they did not germinate on normal stigmas. The 1:1 segregation of fertile and sterile plants in a BC1F1 population from a cross between W1-R and a maintainer line demonstrated that fertility restoration is controlled by a single gene. The fertile seed set of all the F2 plants examined indicated that the fertility restoration functions gametophytically. We designated the fertility restorer gene Rfcw. Using cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR) markers, we localized Rfcw to chromosome 4 with a genetic distance of 0.6 cM from the nearest SSR marker.

本文言語English
ページ(範囲)696-701
ページ数6
ジャーナルTheoretical and Applied Genetics
111
4
DOI
出版ステータスPublished - 2005 8 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 農業および作物学
  • 遺伝学

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