Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

Osamu Tanabe, Gloria A. Gomez, Yasumasa Nishito, Hirofumi Usui, Masao Takeda

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Two cDNAs for possible splicing variants of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A, were isolated. These variants were identified from human cerebral cortex by library screening and PCR, and designated δ1 and δ3 isoforms, while the previously reported isoform [Tanabe et al. (1996) FEBS Lett. 379, 107-111] was designated δ2. Compared with the δ2 isoform, the δ1 isoform contained a 32-residue insertion beginning at residue δ4, and consisted of 602 amino acids in all. The δ3 isoform lacked a 74-residue sequence corresponding to residues 1083 of the δ2 isoform, and consisted of 496 amino acids. Using isoform-specific antipeptide antisera, the 74-kDa subunit (B″ or δ) originally purified from human erythrocytes was identified as the δ1 isoform.

本文言語English
ページ(範囲)52-56
ページ数5
ジャーナルFEBS Letters
408
1
DOI
出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

フィンガープリント

「Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル