Molecular cloning of novel Monad binding protein containing tetratricopeptide repeat domains

Yuki Itsuki, Makio Saeki, Hirokazu Nakahara, Hiroshi Egusa, Yasuyuki Irie, Yutaka Terao, Shigetada Kawabata, Hirofumi Yatani, Yoshinori Kamisaki

研究成果: Article査読

29 被引用数 (Scopus)

抄録

We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-α(TNF-α) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpression of RPAP3 in HEK 293 potentiated caspase-3 activation and apoptosis induced by TNF-α and CHX. In addition, knockdown of RPAP3 by RNA interference resulted in a significant reduction of apoptosis induced by TNF-α and CHX in HEK293 and HeLa cells. These results raise the possibility that RPAP3, together with Monad, may function as a novel modulator of apoptosis pathway. Structured summary: MINT-6551090:Monad (uniprotkb:Q96MX6) physically interacts (MI:0218) with RPAP3 (uniprotkb:Q9H6T3) by anti tag coimmunoprecipitation (MI:0007)MINT-6551101, MINT-6551118:Monad (uniprotkb:Q96MX6) physically interacts (MI:0218) with RPAP3 (uniprotkb:Q9H6T3) by pull down (MI:0096)MINT-6551132:RPAP3 (uniprotkb:Q9H6T3) physically interacts (MI:0218) with Monad (uniprotkb:Q96MX6) by anti bait coimmunoprecipitation (MI:0006).

本文言語English
ページ(範囲)2365-2370
ページ数6
ジャーナルFEBS Letters
582
16
DOI
出版ステータスPublished - 2008 7月 9
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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