Molecular Cloning of cDNA to mRNA for a Cerebellar Spot 35 Protein

T. Yamakuni, R. Kuwano, S. Odani, N. Miki, K. Yamaguchi, Y. Takahashi

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Abstract: The nucleotide sequence of mRNA for rat cerebellar spot 35 protein, a Ca‐binding protein, was determined from recombinant complementary DNA (cDNA) clones. The sequence was composed of 1, 714 base pairs (bp) which included the 783 bp of the complete coding region, the 130 bp of the 5′‐noncoding region, and the 801 bp of the 3‐noncoding region containing a polyadenylation signal. In addition, a polyadenylic acid [poly(A)] tail was also found. Because the size of spot 35 mRNA was estimated to be about 1,900 bases by Northern blot analysis, the longest insert was verified to contain a nearly full‐length cDNA sequence including the poly(A) tail. The amino acid sequence of the protein deduced from the nucleotide sequence contains 261 amino acids and at least five Ca‐binding domains. There was a high homology in the amino acid sequences (79%) and the nucleotide sequences (77%) between spot 35 protein and chick intestinal Ca‐binding protein (28K).

本文言語English
ページ(範囲)1590-1596
ページ数7
ジャーナルJournal of Neurochemistry
48
5
DOI
出版ステータスPublished - 1987 5月

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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