TY - JOUR
T1 - Molecular cloning of cDNA of S100α subunit mRNA
AU - Kuwano, Ryozo
AU - Maeda, Toshinaga
AU - Usui, Hiroshi
AU - Araki, Kazuaki
AU - Yamakuni, Toru
AU - Ohshima, Yuka
AU - Kurihara, Tadashi
AU - Takahashi, Yasuo
N1 - Funding Information:
This work was supportedi n part by a Grant-in-Aid for Scientific Researchf rom the Ministry of Education, Sciencea nd Culture of Japan to Y.T.
PY - 1986/6/23
Y1 - 1986/6/23
N2 - The primary structure of the bovine S-100α mRNA on the basis of molecular cloning and sequence analysis of the cDNA are described. The sequence is composed of 532 bp which include the 282 bp of the complete coding region, 89 bp at the 5'-noncoding region, 161 bp at the 3'-noncoding region, polyadenylation signal, ATTAAA and poly(A) tail. Northern blot analysis shows that the size of S-100α mRNA is about 700-800 bases long and a single mRNA occurs in bovine brain. Bovine brain contains both S100α and β subunits and their mRNAs. In contrast, the rat brain contains only S 100α subunit and its mRNA.
AB - The primary structure of the bovine S-100α mRNA on the basis of molecular cloning and sequence analysis of the cDNA are described. The sequence is composed of 532 bp which include the 282 bp of the complete coding region, 89 bp at the 5'-noncoding region, 161 bp at the 3'-noncoding region, polyadenylation signal, ATTAAA and poly(A) tail. Northern blot analysis shows that the size of S-100α mRNA is about 700-800 bases long and a single mRNA occurs in bovine brain. Bovine brain contains both S100α and β subunits and their mRNAs. In contrast, the rat brain contains only S 100α subunit and its mRNA.
KW - S-100 protein mRNA cDNA cloning Nudeotide sequence
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U2 - 10.1016/0014-5793(86)80656-1
DO - 10.1016/0014-5793(86)80656-1
M3 - Article
C2 - 3755105
AN - SCOPUS:0022468812
VL - 202
SP - 97
EP - 101
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -