Molecular cloning, characterization and expression of an elongation factor 1α gene in maize

Thomas Berberich, Kazuyuki Sugawara, Mariko Harada, Tomonobu Kusano

    研究成果: Article査読

    57 被引用数 (Scopus)

    抄録

    A cDNA (zmEF1A) and the corresponding genomic clone (zmgEF1A) of a member of the gene family encoding the α subunit of translation elongation factor 1 (EF-1α) have been isolated from maize. The deduced amino acid sequence is 447 residues long interrupted by one intron. Southern blot analysis reveals that the cloned EF-1α gene is one member out of a family consisting of at least six genes. As shown by northern hybridizations in leaves the mRNA level increases at low temperature whereas time-course experiments over 24 h at 5°C show that in roots the overall mRNA level of EF-1α is transiently decreased. These results indicate that the expression of EF-1α is differently regulated in leaves and roots under cold stress.

    本文言語English
    ページ(範囲)611-615
    ページ数5
    ジャーナルPlant Molecular Biology
    29
    3
    DOI
    出版ステータスPublished - 1995 11 1

    ASJC Scopus subject areas

    • Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)

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