Molecular Cloning and Intrarenal Localization of Rat Prostaglandin E2 Receptor EP3 Subtype

Kazuhisa Takeuchi, Takaaki Abe, Nobuyuki Takahashi, Keishi Abe

研究成果: Article査読

65 被引用数 (Scopus)

抄録

A rat kidney cDNA library was screened with a mouse prostaglandin (PG) E2 receptor EP subtype cDNA as a probe, and a 2.2-kilobase pair cDNA was isolated. The cDNA encodes 365 amino acids with 97.0% sequence identity to mouse EP receptor. Specific binding of [3H]PGE2 was found in COS-7 cells transfected with the cDNA (Kd=3.2nM) and was displaced with unlabeled prostaglandins in the order of PGE2 = PGE1 >> PGF > PGD2. Thus, a cDNA for rat EP3 (rEP3) receptor was cloned. In situ hybridization revealed expression of rEP3 receptor principally in tubules of renal medulla. Consistent with this observation, the reverse transcription and polymerase chain reaction (RT-PCR) technique using dissected nephron segments showed the receptor expression specifically in medullary thick ascending limbs of Henle’s loop (mTAL), cortical TAL (cTAL), cortical collecting ducts (CCD) and inner medullary collecting ducts (IMCD), indicating a possible diuretic and natriuretic role of rEP3 receptor at the distal nephron segments.

本文言語English
ページ(範囲)885-891
ページ数7
ジャーナルBiochemical and biophysical research communications
194
2
DOI
出版ステータスPublished - 1993 7 30

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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