Molecular cloning and expression of Thiobacillus ferrooxidans chromosomal ribulose bisphosphate carboxylase genes in Escherichia coli

Tomonobu Kusano, Kazuyuki Sugawara, Chihiro Inoue, Nobuhiro Suzuki

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The genes coding for d-ribulose-1,5-bisphosphate carboxylase (RuBPCase) from an iron-oxidizing bacterium, Thiobacillus ferrooxidans, were cloned into an Escherichia coli plasmid, pUC18. The recombinant plasmid, termed pTR11, contained a 4.0-kb PstI fragment including the entire coding regions for both large and small subunits of RuBPCase. Escherichia coli carrying pTR11 did not show any CO2-fixing activity. However, a derivative plasmid with an appropriate deletion, which was placed under the control of a tac promoter, conferred ribulose bisphosphate-dependent CO2-fixing activity on the host cell. Analysis of gel-filtration chromatography of the RuBPCase synthesized in E. coli revealed that it had a hexadecameric form like the native enzyme of T. ferrooxidans.

本文言語English
ページ(範囲)35-41
ページ数7
ジャーナルCurrent Microbiology
22
1
DOI
出版ステータスPublished - 1991 1月
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 応用微生物学とバイオテクノロジー

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