Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcβ1-6(GlcNAcβ1-2)-Manα1-R[GlcNAc to Man]β1,4N-acetylglucosaminyltransferase VI

Y. Sakamoto, T. Taguchi, K. Honke, H. Korekane, H. Watanabe, Y. Tano, N. Dohmae, K. Takio, A. Horii, N. Taniguchi

研究成果: Article

11 引用 (Scopus)

抜粋

A cDNA that encodes UDP-N-acetyl-D-glucosamine (GlcNAc):GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R[GlcNAc to Man]β1,4N-acetylglucosaminyltransferase VI (GnT VI), which is responsible for the formation of pentaantennary asparagine-linked oligosaccharides (N-glycans), has been cloned from a hen oviduct cDNA library based on the partial amino acid sequences of the purified enzyme. The isolated cDNA clone contained an open reading frame encoding 464 amino acids, including all of the peptides that were sequenced. The deduced amino acid sequence predicts a type II transmembrane topology and contains two potential N-glycosylation sites. The primary structure was found to be significantly similar to human GnT IV-homologue, the gene for which was cloned from the deleted region in pancreatic cancer, and to human and bovine GnT IVs. Chicken GnT VI-transfected COS-1 cells showed a high GnT VI activity (26.8 pmol/h/mg protein), whereas nontransfected, mocktransfected, or human GnT IV-homologue-transfected COS-1 cells had no activity. Northern blot analysis using poly(A)+ RNA from hen oviduct indicated that the size of GnT VI mRNA is 2.1 kilobases. Reverse transcription-polymerase chain reaction analysis showed that GnT VI mRNA was relatively highly expressed in oviduct, spleen, lung, and colon.

元の言語English
ページ(範囲)36029-36034
ページ数6
ジャーナルJournal of Biological Chemistry
275
発行部数46
DOI
出版物ステータスPublished - 2000 11 17

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcβ1-6(GlcNAcβ1-2)-Manα1-R[GlcNAc to Man]β1,4N-acetylglucosaminyltransferase VI' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用