Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.)

Jong In Park, Hirokazu Hakozaki, Makoto Endo, Yoshinobu Takada, Hitoshi Ito, Masanori Uchida, Tomihiro Okabe, Masao Watanabe

研究成果: Article査読

33 被引用数 (Scopus)

抄録

In our previous cDNA microarray analysis, we identified 53 mature anther-specific genes, whose function was unknown, in rice. We reanalyzed these genes from the viewpoint of the specific amino acid motif. Out of 53 genes, three genes, Os-26, Os-32, and Os-169 (renamed as OsSCP1, OsSCP2, and OsSCP3), encoded cysteine-rich motif (Cys-X3-Cys-X13-Cys-X3-Cys), indicating that they were novel small cysteine-rich proteins. From the search of specific elements in promoter regions, several pollen-specific elements were found. In order to determine whether three promoters were functional in pollen or not, the gene constructs with promoter regions fused to the β-glucuronidase gene were transformed into tobacco. Histochemical analysis showed that these promoters were active in the mature pollen grains and pollen tubes. Furthermore, OsSCP1 and OsSCP3 formed a multigene family tandemly in the rice genome. From the results, OsSCPs might have important roles in mature pollen development and pollen tube growth.

本文言語English
ページ(範囲)466-474
ページ数9
ジャーナルPlant Cell Reports
25
5
DOI
出版ステータスPublished - 2006 5

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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