TY - JOUR
T1 - Molecular characterization of anaerobic sulfur-oxidizing microbial communities in up-flow anaerobic sludge blanket reactor treating municipal sewage
AU - Aida, Azrina A.
AU - Hatamoto, Masashi
AU - Yamamoto, Masamitsu
AU - Ono, Shinya
AU - Nakamura, Akinobu
AU - Takahashi, Masanobu
AU - Yamaguchi, Takashi
N1 - Funding Information:
The part of this work was supported by the Japan Society for the Promotion of Science (JSPS) Grant Number 21676004 and by the Japan Science and Technology Agency (JST) , Science and Technology Research Partnership for Sustainable Development (SATREPS).
Publisher Copyright:
© 2014 The Society for Biotechnology, Japan.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - A novel wastewater treatment system consisting of an up-flow anaerobic sludge blanket (UASB) reactor and a down-flow hanging sponge (DHS) reactor with sulfur-redox reaction was developed for treatment of municipal sewage under low-temperature conditions. In the UASB reactor, a novel phenomenon of anaerobic sulfur oxidation occurred in the absence of oxygen, nitrite and nitrate as electron acceptors. The microorganisms involved in anaerobic sulfur oxidation have not been elucidated. Therefore, in this study, we studied the microbial communities existing in the UASB reactor that probably enhanced anaerobic sulfur oxidation. Sludge samples collected from the UASB reactor before and after sulfur oxidation were used for cloning and terminal restriction fragment length polymorphism (T-RFLP) analysis of the 16S rRNA genes of the bacterial and archaeal domains. The microbial community structures of bacteria and archaea indicated that the genus Smithella and uncultured bacteria within the phylum Caldiserica were the dominant bacteria groups. Methanosaeta spp. was the dominant group of the domain archaea. The T-RFLP analysis, which was consistent with the cloning results, also yielded characteristic fingerprints for bacterial communities, whereas the archaeal community structure yielded stable microbial community. From these results, it can be presumed that these major bacteria groups, genus Smithella and uncultured bacteria within the phylum Caldiserica, probably play an important role in sulfur oxidation in UASB reactors.
AB - A novel wastewater treatment system consisting of an up-flow anaerobic sludge blanket (UASB) reactor and a down-flow hanging sponge (DHS) reactor with sulfur-redox reaction was developed for treatment of municipal sewage under low-temperature conditions. In the UASB reactor, a novel phenomenon of anaerobic sulfur oxidation occurred in the absence of oxygen, nitrite and nitrate as electron acceptors. The microorganisms involved in anaerobic sulfur oxidation have not been elucidated. Therefore, in this study, we studied the microbial communities existing in the UASB reactor that probably enhanced anaerobic sulfur oxidation. Sludge samples collected from the UASB reactor before and after sulfur oxidation were used for cloning and terminal restriction fragment length polymorphism (T-RFLP) analysis of the 16S rRNA genes of the bacterial and archaeal domains. The microbial community structures of bacteria and archaea indicated that the genus Smithella and uncultured bacteria within the phylum Caldiserica were the dominant bacteria groups. Methanosaeta spp. was the dominant group of the domain archaea. The T-RFLP analysis, which was consistent with the cloning results, also yielded characteristic fingerprints for bacterial communities, whereas the archaeal community structure yielded stable microbial community. From these results, it can be presumed that these major bacteria groups, genus Smithella and uncultured bacteria within the phylum Caldiserica, probably play an important role in sulfur oxidation in UASB reactors.
KW - Anaerobic sulfur oxidation
KW - Microbial community
KW - Molecular analysis
KW - Up-flow anaerobic sludge blanket
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=84927175755&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84927175755&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2014.04.011
DO - 10.1016/j.jbiosc.2014.04.011
M3 - Article
C2 - 24930844
AN - SCOPUS:84927175755
VL - 118
SP - 540
EP - 545
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -