Modified sympathetic regulation in N-type calcium channel null-mouse

Manabu Murakami, Takayoshi Ohba, Tsai Wen Wu, Susumu Fujisawa, Takashi Suzuki, Yoichiro Takahashi, Eiki Takahashi, Hiroyuki Watanabe, Ichiro Miyoshi, Kyoichi Ono, Hironobu Sasano, Hiroshi Ito, Toshihiko Iijima

研究成果: Article査読

12 被引用数 (Scopus)

抄録

To elucidate the physiological importance of neuronal (N)-type calcium channels in sympathetic controls, we analyzed N-type channel-deficient (NKO) mice. Immunoprecipitation analysis revealed increased interaction between β3 (a major accessory subunit of N-type channels) and R-type channel-forming CaV2.3 in NKO mice. R-R intervals in NKO ECG recordings were elongated and fluctuating, suggesting disturbed sympathetic tonus. N-type channel inhibitors elongated the R-R interval in control mice, whereas R-type channel blocking with SNX-482 significantly affected NKO but not control mice, indicating a compensatory role for R-type channels. Echocardiography and Langendorff heart analysis confirmed a major role for R-type channels in NKO mice. Combined, our biochemical and physiological analyses strongly suggest that the remaining sympathetic tonus in NKO mice is dependent on R-type calcium channels.

本文言語English
ページ(範囲)1016-1020
ページ数5
ジャーナルBiochemical and biophysical research communications
354
4
DOI
出版ステータスPublished - 2007 3 23

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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