TY - JOUR
T1 - Moderate dependence of reactive oxygen species production on membrane potential in avian muscle mitochondria oxidizing glycerol 3-phosphate
AU - Kikusato, Motoi
AU - Toyomizu, Masaaki
N1 - Funding Information:
Funding This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI grant nos. 90613042 (M.K.) and 24380147 (M.T.).
Publisher Copyright:
© 2015, The Physiological Society of Japan and Springer Japan.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Mitochondria are a major source of reactive oxygen species production in cells, and the production level is sensitive to the magnitude of the membrane potential (ΔΨ). The present study investigated the level of superoxide production in mitochondria oxidizing glycerol 3-phosphate (GP) and its dependence on ΔΨ in isolated avian muscle mitochondria. The levels of superoxide produced in mitochondria oxidizing GP were lower than those obtained with succinate and were similar to those obtained with NADH-linked substrates (glutamate/malate/pyruvate). The dependence of superoxide production on ΔΨ in mitochondria oxidizing GP was lower than that of mitochondria oxidizing succinate, and a weak dependence of GP-supported superoxide production on ΔΨ was observed in the presence of NADH-linked substrates or succinate. These results suggest that the levels of superoxide generated in response to GP are quantitatively low, but they are unsusceptible to changes in ΔΨ in avian muscle mitochondria.
AB - Mitochondria are a major source of reactive oxygen species production in cells, and the production level is sensitive to the magnitude of the membrane potential (ΔΨ). The present study investigated the level of superoxide production in mitochondria oxidizing glycerol 3-phosphate (GP) and its dependence on ΔΨ in isolated avian muscle mitochondria. The levels of superoxide produced in mitochondria oxidizing GP were lower than those obtained with succinate and were similar to those obtained with NADH-linked substrates (glutamate/malate/pyruvate). The dependence of superoxide production on ΔΨ in mitochondria oxidizing GP was lower than that of mitochondria oxidizing succinate, and a weak dependence of GP-supported superoxide production on ΔΨ was observed in the presence of NADH-linked substrates or succinate. These results suggest that the levels of superoxide generated in response to GP are quantitatively low, but they are unsusceptible to changes in ΔΨ in avian muscle mitochondria.
KW - Carbonyl cyanide p-trifluoromethoxyphenyl hydrazine
KW - Mitochondrial glycerol 3-phosphate dehydrogenase
KW - Mitochondrial membrane potential
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U2 - 10.1007/s12576-015-0395-2
DO - 10.1007/s12576-015-0395-2
M3 - Article
C2 - 26335765
AN - SCOPUS:84945460689
VL - 65
SP - 555
EP - 559
JO - The Journal of Physiological Sciences
JF - The Journal of Physiological Sciences
SN - 1880-6546
IS - 6
ER -