Mitogenic Activity of Cytoplasmic Membranes Isolated from L-Forms of Staphylococcus aureus

Haruhiko Takada, Yoshiyuki Hirachi, Hideo Hashizume, Shozo Kotani

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Cytoplasmic membranes of L-forms of Staphylococcus aureus exerted a strong mitogenic effect on splenocytes of athymic nude mice as well as normal mice, while a cytoplasmic fraction of the same bacteria did not show definite mitogenicity. The mitogenic principle(s) of the membrane fraction was resistant to treatment with trypsin and was heat stable (at 100 C for 10 min). The active principle (s) in the insoluble residue of the membrane fraction digested with trypsin was not extracted with cold acetone, but could be solubilized by extraction with a cold chloroform-methanol mixture (2:1, v/v). The mitogenic principle(s) in the extract was fractionated by silicic acid column chromatography. Among five fractions separated by chromatography, fractions eluted with chloroform-methanol mixtures (1:1 and 1:20, v/v) were found to be strongly mitogenic. The cytoplasmic membranes of the L-forms also exerted a definite mitogenic effect on guinea pig splenocytes, but not on the thymocytes.

本文言語English
ページ(範囲)1079-1090
ページ数12
ジャーナルMICROBIOLOGY and IMMUNOLOGY
24
11
DOI
出版ステータスPublished - 1980 1 1

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • ウイルス学

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