Minimal nucleotide duplex formation in water through enclathration in self-assembled hosts

Tomohisa Sawada, Michito Yoshizawa, Sota Sato, Makoto Fujita

    研究成果: Article査読

    176 被引用数 (Scopus)

    抄録

    Short nucleotide fragments such as mono- and dinucleotides are generally unable to form stable hydrogen-bonded base pairs or duplexes in water. Within the hydrophobic pockets of enzymes, however, even short fragments form stable duplexes to transmit genetic information. Here, we demonstrate the efficient formation of hydrogen-bonded base pairs from mononucleotides in water through enclathration in the hydrophobic cavities of self-assembled cages. Crystallographic studies and 1H- and 15N-NMR spectroscopy clearly reveals pair-selective recognition of mononucleotides and the selective formation of an anti-Hoogsteen-type base pair in the cage's cavity. Within an analogous expanded cage, dinucleotides are also found to form a stable duplex in water. These results emphasize how hydrogen-bonded base pairing is amplified in a local hydrophobic area isolated from aqueous solution.

    本文言語English
    ページ(範囲)53-56
    ページ数4
    ジャーナルNature Chemistry
    1
    1
    DOI
    出版ステータスPublished - 2009 4月

    ASJC Scopus subject areas

    • 化学 (全般)
    • 化学工学(全般)

    フィンガープリント

    「Minimal nucleotide duplex formation in water through enclathration in self-assembled hosts」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル