We have been studying cooperative distributed antenna transmission (CDAT) as a promising technique for realizing the 5th generation (5G) high-speed mobile communications. Assuming time division duplex (TDD), the macro-cell base station (MBS) and the user equipments (UEs) conduct the channel estimation independently to obtain the shared channel state information (CSI). In this paper, we introduce a TDD frame structure consisting of uplink pilot time slot and downlink pilot time slot followed by data time slots. MIMO channel estimation is done before the user data transmission. Also presented is the pilot signal design. The accuracy of the proposed MIMO channel estimation and achievable bit error rate (BER) performance of user data transmission are evaluated by computer simulation.