Microspots of GOD-HRP bienzyme for scanning chemiluminescence microscopy with higher resolution

Yu Hirano, Shigenobu Kasai, Matsuhiko Nishizawa, Tomokazu Matsue

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Scanning chemiluminescence microscopy (SCLM) was applied to visualize bienzyme reaction at a microspot (50 μm in radius) composed of glucose oxidase (GOD) and horseradish peroxidase (HRP). In an aqueous solution containing glucose, a glass capillary tip was scanned over the bienzyme spot with luminol injected continuously from the tip to induce local chemiluminescence. The GOD catalyzes the oxidation of glucose by oxygen to form H2O2 which oxidizes luminol in the presence of HRP to generate chemiluminescence. Since the enzymatic luminescence reaction proceeds only within a bienzyme spot, even an array of spots was clearly imaged by SCLM without any significant interference between neighbor spots.

本文言語English
ページ(範囲)946-948
ページ数3
ジャーナルElectrochemistry
69
12
DOI
出版ステータスPublished - 2001 12

ASJC Scopus subject areas

  • 電気化学

フィンガープリント

「Microspots of GOD-HRP bienzyme for scanning chemiluminescence microscopy with higher resolution」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル