Micropatterning with different cell types by dielectrophoretic manipulation

Tomoyuki Yasukawa, Masato Suzuki, Hitoshi Shiku, Tomokazu Matsue

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

In this paper, a versatile, rapid and reproducible method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent microelectrode subunits was fabricated with indium-tin-oxide (ITO) and used as a template to form cellular micropatterns. A suspension of C2C12 cells was introduced into the device between the upper slide and the bottom IDA. In the present system, the n-DEP force is induced by applying an ac voltage (typically 12 Vpp, 1 MHz) to direct cells toward a weaker region of electric field strength. The cells aligned above one of the bands of IDA within 1 min since the aligned areas on the slide were regions with the lower electric field. The application of an ac voltage for 5 min allows the cells to adsorb onto the cell culture slide. After disassembling the device and removing excess cells, the culture slide was assembled again with the IDA electrode, and was rotated 90° relative to the previous setup. The second cell type was patterned in lines using the same method as with the first set of cells, forming a grid pattern on the slide.

本文言語English
ホスト出版物のタイトル2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
ページ179-182
ページ数4
DOI
出版ステータスPublished - 2007
イベント2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS - Nagoya, Japan
継続期間: 2007 11 112007 11 14

出版物シリーズ

名前2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS

Other

Other2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
国/地域Japan
CityNagoya
Period07/11/1107/11/14

ASJC Scopus subject areas

  • 人工知能
  • コンピュータ ネットワークおよび通信
  • 人間とコンピュータの相互作用
  • 電子工学および電気工学

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