Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography

Fumitaka Mafuné, Ken Miyajima, Kensuke Tono, Yoshihiro Takeda, Jun Ya Kohno, Naoya Miyauchi, Jun Kobayashi, Yasumasa Joti, Eriko Nango, So Iwata, Makina Yabashi

研究成果: Article査読

33 被引用数 (Scopus)

抄録

A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 Å from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.

本文言語English
ページ(範囲)520-523
ページ数4
ジャーナルActa Crystallographica Section D: Structural Biology
72
4
DOI
出版ステータスPublished - 2016
外部発表はい

ASJC Scopus subject areas

  • Structural Biology

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