Microbial diversity in an iron oxidation tank of an AMD treatment plant at an abandoned sulphur mine

Koichi Suto, Hernando Bacosa, Chihiro Inoue, Eiji Matsushima

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

This paper describes about microbial diversity in an iron oxidation tank of an AMD treatment plant established at an abandoned sulphur mine in Japan. Since the mining operation was stopped, this mine has produced strong acidic mine drainage, pH 1.8, including a high concentration of ferrous iron, 301mg/L, and the flow rate was about 4m3/min. In 2006, a pilot scale microbial iron oxidizing system was installed to remove total iron more easily from the AMD by oxidizing ferrous iron to ferric iron. From the start of this pilot operation, microbial diversity in the iron oxidation tank was investigated using a PCR-DGGE method for about two months. In the PCR, V3 region of 16S rRNA gene for Bacteria was amplified. The profiles of DGGE showed that there were three dominant species in the iron oxidation tank through the experimental period. Number of bands on DGGE profiles decreased with dates of sampling so that the microbial population became less diverse because of the iron oxidizing operation. There was a wide variety of bacterial species of even though conditions were strongly acidic.

本文言語English
ホスト出版物のタイトルBiohydrometallurgy
ホスト出版物のサブタイトルFrom the Single Cell to the Environment
出版社Trans Tech Publications
ページ493-496
ページ数4
ISBN(印刷版)0878494529, 9780878494521
DOI
出版ステータスPublished - 2007
イベント17th International Biohydrometallurgy Symposium, IBS 2007 - Frankfurt am Main, Germany
継続期間: 2007 9 22007 9 5

出版物シリーズ

名前Advanced Materials Research
20-21
ISSN(印刷版)1022-6680

Other

Other17th International Biohydrometallurgy Symposium, IBS 2007
CountryGermany
CityFrankfurt am Main
Period07/9/207/9/5

ASJC Scopus subject areas

  • Engineering(all)

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