Microarray analysis reveals distinct signaling pathways transcriptionally activated by infection with bovine viral diarrhea virus in different cell types

Daisuke Yamane, Muhammad Atif Zahoor, Yassir Mahgoub Mohamed, Walid Azab, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Infection with bovine viral diarrhea virus (BVDV) causes different effects depending on its biotype in vitro; cytopathogenic (cp) strains induce apoptosis, type I interferon (IFN), and various stress-mediated responses, whereas non-cytopathogenic (ncp) strains do not. However, comprehensive transcriptional profiles of the cells infected with BVDV are still unknown. Here we performed microarray analysis of BVDV-infected MDBK epithelial cells and bovine fetal muscle (BFM) fibroblast cells. Infection of both cell types with cp BVDV, but not ncp BVDV, stimulated marked up-regulation of numerous genes belonging to diverse functional classes. However, the pattern of gene expression detected in both cell types was highly distinct from each other. Notably, upon cp BVDV infection, BFM cells exhibited marked induction of IFN-stimulated genes (ISGs), whereas MDBK cells characteristically up-regulated endoplasmic reticulum stress-inducible genes, such as tribbles homolog 3 (TRB3), CHOP and asparagine synthase, and showed much weaker induction of ISGs than BFM cells. This study highlights unexpected diversity in the response of different cell types to BVDV infection.

本文言語English
ページ(範囲)188-199
ページ数12
ジャーナルVirus Research
142
1-2
DOI
出版ステータスPublished - 2009 6
外部発表はい

ASJC Scopus subject areas

  • ウイルス学
  • 感染症
  • 癌研究

フィンガープリント

「Microarray analysis reveals distinct signaling pathways transcriptionally activated by infection with bovine viral diarrhea virus in different cell types」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル