Mercuric ion uptake by escherichia coli cells producing thiobacillus ferrooxidans merC

Chihiro Inoue, Tomonobu Kusano, Simon Silver

研究成果: Article査読

21 被引用数 (Scopus)

抄録

The merC gene of Thiobacillus ferrooxidans was overexpressed in Escherichia coli under the control of the tac promoter. MerC protein synthesized in E. coli has a N-terminal amino acid sequence of S-A-I-X-R-I-I-D-K-I-G-I-V-G-, which agrees with the amino acid sequence deduced from its nucleotide sequence except that an initiating methionine residue was removed. The MerC protein was localized in the particulate (membrane) cell fraction, and not in the soluble cytoplasmic fraction. E. coli cells carrying a plasmid containing the tac promoter-directed merC showed 203Hg2+ uptake in an isopropyl-1-thio-β-d-galactopyranoside (IPTG)-dependent manner.

本文言語English
ページ(範囲)1289-1292
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
60
8
DOI
出版ステータスPublished - 1996 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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