Mechanisms of Action of Growth Hormone-Releasing Peptide-2 in Bovine Pituitary Cells

S. G. Roh, M. L. He, N. Matsunaga, S. Hidaka, H. Hidari

研究成果: Article査読

15 被引用数 (Scopus)

抄録

We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-βNal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture. Doses of GHRP-2 from 10-13 to 10-7 M increased (P < .05) GH secretion. The GHRP-2 (10-7 M) and GH-releasing factor (GRF; 10-7 M) administered together had an additive effect on the release of GH (P < .05). Somatostatin (1 μM) decreased GH secretion in response to GHRP-2 and(or) GRF (P < .05). Secretion of GH in response to GHRP-2 was blocked (P < .01) by a GRF receptor antagonist (.1 μM). Nifedipine (10 μM), a voltage-dependent Ca 2+ channel blocker, inhibited (P < .01) GHRP-2-stimulated GH release. The GH release in response to GHRP-2 and 4β-phorbol-12-myristate-13-acetate (10-7 M), a protein kinase C activator, was additive (P < .01). Forskolin (30 μM), a cAMP elevating agent, further stimulated (P < .01) the GH release in response to GHRP-2. Bovine GH concentrations in culture media were assayed by indirect competitive enzymeimmunoassay. These results showed that GHRP-2 1) stimulates GH secretion from bovine pituitary cells, 2) may partially act via GRF receptor, 3) has GH secretion activity caused by Ca2+ influx via Ca2+ channels, and 4) may increase GH secretion via protein kinase C and cAMP pathways.

本文言語English
ページ(範囲)2744-2748
ページ数5
ジャーナルJournal of animal science
75
10
DOI
出版ステータスPublished - 1997 10月
外部発表はい

ASJC Scopus subject areas

  • 食品科学
  • 動物科学および動物学
  • 遺伝学

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