Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells

Naoto Fukuno; Hiroyuki Matsui; Osamu Suzuki; Keiichi Sasaki; Takayasu Kobayashi; Shinri Tamura

doi:10.1109/MHS.2009.5351995

Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells

Naoto Fukuno, Hiroyuki Matsui, Osamu Suzuki, Keiichi Sasaki, Takayasu Kobayashi, Shinri Tamura

研究成果: Conference contribution

抄録

Mechanical stress plays an essential role in bone homeostasis by regulationg both bone formation and resorption. We have previously shown that cyclic stretch loading to MC3T3-E1 cells induced the expression of two bone remodeling related genes, Fn14 and MCP-3, through activation of ASK1 (a MAPKKK)/JNK and ASK1/p38 pathways, respectively. Here we show that cyclic stretch loading also induced the activation of TAK1 (another MAPKKK). Using TAK1 specific inhibitor 5Z-7 oxozeaenol, we revealed that TAK1 was required for the activation of at least four downstream pathways, namely, JNK, p38, NF-κB and NLK pathways. Moreover, TAK1 is activated via Ca²⁺-CaMKII pathway, resembling the non-canonical Wnt signaling pathway. These observations raise the possibility that TAK1 also plays various essential roles in mechanical stess-induced bone remodeling by activating diverse downstream pathways.

本文言語	English
ホスト出版物のタイトル	20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
ページ	383-384
ページ数	2
DOI	https://doi.org/10.1109/MHS.2009.5351995
出版ステータス	Published - 2009 12 1
イベント	20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science - Nagoya, Japan 継続期間: 2009 11 8 → 2009 11 11

出版物シリーズ

名前	20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science

Other

Other	20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science
Country	Japan
City	Nagoya
Period	09/11/8 → 09/11/11

ASJC Scopus subject areas

Computer Science(all)
Control and Systems Engineering
Electrical and Electronic Engineering

文献へのアクセス

10.1109/MHS.2009.5351995

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引用スタイル

Fukuno, N., Matsui, H., Suzuki, O., Sasaki, K., Kobayashi, T., & Tamura, S. (2009). Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells. In 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science (pp. 383-384). [5351995] (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science). https://doi.org/10.1109/MHS.2009.5351995

Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells. / Fukuno, Naoto; Matsui, Hiroyuki; Suzuki, Osamu ; Sasaki, Keiichi ; Kobayashi, Takayasu; Tamura, Shinri.

20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. p. 383-384 5351995 (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science).

研究成果: Conference contribution

Fukuno, N, Matsui, H, Suzuki, O , Sasaki, K , Kobayashi, T & Tamura, S 2009, Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells. in 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science., 5351995, 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science, pp. 383-384, 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science, Nagoya, Japan, 09/11/8. https://doi.org/10.1109/MHS.2009.5351995

Fukuno N, Matsui H, Suzuki O , Sasaki K , Kobayashi T, Tamura S. Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells. In 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. p. 383-384. 5351995. (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science). https://doi.org/10.1109/MHS.2009.5351995

Fukuno, Naoto ; Matsui, Hiroyuki ; Suzuki, Osamu ; Sasaki, Keiichi ; Kobayashi, Takayasu ; Tamura, Shinri. / Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells. 20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science. 2009. pp. 383-384 (20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science).

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abstract = "Mechanical stress plays an essential role in bone homeostasis by regulationg both bone formation and resorption. We have previously shown that cyclic stretch loading to MC3T3-E1 cells induced the expression of two bone remodeling related genes, Fn14 and MCP-3, through activation of ASK1 (a MAPKKK)/JNK and ASK1/p38 pathways, respectively. Here we show that cyclic stretch loading also induced the activation of TAK1 (another MAPKKK). Using TAK1 specific inhibitor 5Z-7 oxozeaenol, we revealed that TAK1 was required for the activation of at least four downstream pathways, namely, JNK, p38, NF-κB and NLK pathways. Moreover, TAK1 is activated via Ca2+-CaMKII pathway, resembling the non-canonical Wnt signaling pathway. These observations raise the possibility that TAK1 also plays various essential roles in mechanical stess-induced bone remodeling by activating diverse downstream pathways.",

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