Mechanical characterization of cyanobacteria under osmotic stress

S. Sahuma, D. Chang, F. Arai, K. Kera, N. Uozumi

研究成果: Conference contribution

抄録

We report the evaluation results of mechanical characterization of Synechocystis sp. PCC 6803. We have constructed the force measurement system using the microfluidic chip on the microscope with optical tweezers. Indentation and force sensor probes are integrated into the microfluidic chip. Using the constructed system, we evaluated the Young's modulus of two cell groups of wild type and mutant type, which was knocked out mechanosensitive channels. They are placed in two different mediums; normal and high-osmolality condition. While there was no significant difference of stiffness between two groups in normal medium, the average value and the standard deviation of Young's modulus were different in high-osmolality condition. These results indicate the basic function of mechanosensitive channels, because there was clear relationship between the stiffness and the osmotic stress. We confirmed that the measurement system will contribute to clarify unknown function of ion channels of cyanobacteria based on relationship between environmental stress and mechanical characteristics on single cell level.

本文言語English
ホスト出版物のタイトル2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1248-1251
ページ数4
ISBN(電子版)9781509050789
DOI
出版ステータスPublished - 2017 2月 23
イベント30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States
継続期間: 2017 1月 222017 1月 26

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(印刷版)1084-6999

Other

Other30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
国/地域United States
CityLas Vegas
Period17/1/2217/1/26

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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