Measurement of tryptophan in peptides by acid hydrolysis in the presence of phenol and its application to the amino acid sequence of a sea anemone toxin

Koji Muramoto, Satoshi Sunahara, Hisao Kamiya

    研究成果: Article査読

    33 被引用数 (Scopus)

    抄録

    The addition of phenol (about 1 % to 6 M HCl largely prevented destruction of tryptophan during hydrolysis of pep tides at 1l0°C for 22hr. Tryptophan recovery depended on the volume of 6 M HCl containing phenol and the concentration of phenol. The maximum tryptophan recovery was 85% for a standard amino acid mixture. The recovery was slightly lower for proteins. This hydrolytic procedure was advantageous for micro amino acid analysis using a conventional high performance liquid chromatography with a precolumn labeling technique. The method was used in the amino acid sequence analysis of a minor component of sea anemone toxins isolated from Anthopleura fuscoviridis. The toxin consisted of 48 amino acid residues with three tryptophan residues.

    本文言語English
    ページ(範囲)1607-1616
    ページ数10
    ジャーナルAgricultural and Biological Chemistry
    51
    6
    DOI
    出版ステータスPublished - 1987 1 1

    ASJC Scopus subject areas

    • 生化学、遺伝学、分子生物学(全般)
    • 農業および生物科学(全般)

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