抄録
This paper reports the measurement of the binary mass diffusion coefficient for proteins with a wide range of molecular size. The diffusion coefficient is obtained by conducting diffusion experiments in the dilute region. Transient concentration profiles were measured by a phase-shifting interferometer and subsequently compared with a numerical calculation based on Fick's law to determine the diffusion coefficient. Distilled water was used as solvent in free diffusion experiments conducted at T = (25 ± 1.0)°C. The method was validated by measuring the diffusion coefficient of aqueous NaCl, Sucrose, and BSA, which values have been extensively reported in the literature. The values of the diffusion coefficient for seven proteins: aprotinin (6.5 kDa), α-lactalbumin (14.2 kDa), lysozyme (14.3 kDa), trypsin inhibitor (20.1 kDa), ovalbumin (44.2 kDa), bovine serum albumin (66.7 kDa), and phosphorylase b (97.2 kDa), were determined in the dilute region of 0-3 mg/ml. The results are compared with the Stokes-Einstein equation. The influence of the molecular structure and pH on the diffusion coefficient is discussed.
本文言語 | English |
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ホスト出版物のタイトル | Diffusion in Solids and Liquids VII |
ホスト出版物のサブタイトル | Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials |
出版社 | Trans Tech Publications Ltd |
ページ | 452-458 |
ページ数 | 7 |
巻 | 326-328 |
ISBN(印刷版) | 9783037854006 |
DOI | |
出版ステータス | Published - 2012 |
イベント | 7th International Conference on Diffusion in Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials, DSL 2011 - Algarve, Portugal 継続期間: 2011 6月 26 → 2011 6月 30 |
Other
Other | 7th International Conference on Diffusion in Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials, DSL 2011 |
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国/地域 | Portugal |
City | Algarve |
Period | 11/6/26 → 11/6/30 |
ASJC Scopus subject areas
- 放射線
- 材料科学(全般)
- 凝縮系物理学