Measurement of the molecular mass dependence of the mass diffusion coefficient in protein aqueous solutions

Juan F. Torres, Atsuki Komiya, Junnosuke Okajima, Maruyama Shigenao

研究成果: Conference contribution

6 被引用数 (Scopus)

抄録

This paper reports the measurement of the binary mass diffusion coefficient for proteins with a wide range of molecular size. The diffusion coefficient is obtained by conducting diffusion experiments in the dilute region. Transient concentration profiles were measured by a phase-shifting interferometer and subsequently compared with a numerical calculation based on Fick's law to determine the diffusion coefficient. Distilled water was used as solvent in free diffusion experiments conducted at T = (25 ± 1.0)°C. The method was validated by measuring the diffusion coefficient of aqueous NaCl, Sucrose, and BSA, which values have been extensively reported in the literature. The values of the diffusion coefficient for seven proteins: aprotinin (6.5 kDa), α-lactalbumin (14.2 kDa), lysozyme (14.3 kDa), trypsin inhibitor (20.1 kDa), ovalbumin (44.2 kDa), bovine serum albumin (66.7 kDa), and phosphorylase b (97.2 kDa), were determined in the dilute region of 0-3 mg/ml. The results are compared with the Stokes-Einstein equation. The influence of the molecular structure and pH on the diffusion coefficient is discussed.

本文言語English
ホスト出版物のタイトルDiffusion in Solids and Liquids VII
ホスト出版物のサブタイトルSolids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials
出版社Trans Tech Publications Ltd
ページ452-458
ページ数7
326-328
ISBN(印刷版)9783037854006
DOI
出版ステータスPublished - 2012
イベント7th International Conference on Diffusion in Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials, DSL 2011 - Algarve, Portugal
継続期間: 2011 6 262011 6 30

Other

Other7th International Conference on Diffusion in Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials, DSL 2011
CountryPortugal
CityAlgarve
Period11/6/2611/6/30

ASJC Scopus subject areas

  • Radiation
  • Materials Science(all)
  • Condensed Matter Physics

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