Measurement of the Cell-Body Rotation of Leptospira

研究成果: Chapter

抄録

Leptospira spp. swim in liquid and crawl on surfaces with two periplasmic flagella. The periplasmic flagella attach to the protoplasmic cylinder via basal rotary motors (flagellar motors) and transform the ends of the cell body into spiral or hook shape. The rotations of the periplasmic flagella are thought to gyrate the cell body and rotate the protoplasmic cylinder for propelling the cell; however, the motility mechanism has not been fully elucidated. Since the motility is a critical virulence factor for pathogenic leptospires, the kinematic insight is valuable to understand the mechanism of infection. This chapter describes microscopic methodologies to measure the motility of Leptospira, focusing on rotation of the helical cell body.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ139-148
ページ数10
DOI
出版ステータスPublished - 2020

出版物シリーズ

名前Methods in Molecular Biology
2134
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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